描述:BioLegend公司位于美國加州,致力于為生物醫(yī)藥研究的各個前沿領域提供高質(zhì)量的抗體及其它相關試劑。BioLegend聚集了一批來自PharMingen公司的專業(yè)人士,建立了一支具有雄厚技術背景的專業(yè)隊伍,其中該公司的CEO更是為PharMingen公司的創(chuàng)始人之一。
Reactivity: | All mammalian species |
Formulation: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA). |
Preparation: | The purified protein was conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647. |
Storage & Handling: | The Annexin V solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
Application: | FC - Quality tested |
Recommended Usage: | Each lot of this product is quality control tested by staining of apoptotic cells and analyzing by flow cytometry. The suggested use of this reagent is 5 µl per 1X105 cells in a 100 µl volume of Annexin V Binding Buffer (cat. no. 422201). It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents. |