ESI BIO的使命是成為客戶(hù)信賴(lài)的合作伙伴,將科學(xué)發(fā)現(xiàn)轉(zhuǎn)化為診所。我們通過(guò)提供突破性的干細(xì)胞技術(shù)作為研究級(jí)和治療級(jí)產(chǎn)品來(lái)實(shí)現(xiàn)這一目標(biāo),從研究到診所的照明,以幫助我們的客戶(hù)實(shí)現(xiàn)他們治療疾病的目標(biāo)。
ESI BIO建立在豐富的創(chuàng)新和科學(xué)的基礎(chǔ)之上。我們于2000年在新加坡成立了ES Cell International,在那里我們通過(guò)制造個(gè)可翻譯的臨床級(jí)胚胎干細(xì)胞系來(lái)創(chuàng)建歷史,以便分發(fā)給研究界。
ESIBIO現(xiàn)在開(kāi)展業(yè)務(wù)Alameda CA. 我們是BioTime,Inc。擁有的以干細(xì)胞為基礎(chǔ)的子公司的驕傲家族的成員,我們從中受益于的科學(xué),知識(shí)產(chǎn)權(quán)和干細(xì)胞技術(shù)的深層來(lái)源。
Product Name | SKU | Price |
HyStem® Hydrogel Trial Kit, 2.5 mL | GS310 | $85.00 |
HyStem® Hydrogel Trial Kit w/ PEGSSDA, 2.5 mL | GS310P | $125.00 |
HyStem® Hydrogel Kit, 7.5 mL | GS311 | $225.00 |
HyStem® Hydrogel Kit w/ PEGSSDA, 7.5 mL | GS311P | $250.00 |
HyStem® Hydrogel Kit, 12.5 mL | GS1004 | $275.00 |
HyStem ®水凝膠-的空白石板矩陣。*可定制和無(wú)xeno。組分包括巰基修飾的透明質(zhì)酸(Gycosil ®),巰基反應(yīng)性交聯(lián)劑PEGDA(Extralink ®),并脫氣,去離子水(DG水)。
HyStem ®水凝膠是無(wú)異物的,可*定制。推薦用于需要特定附著因子優(yōu)化以及非貼壁細(xì)胞培養(yǎng),細(xì)胞應(yīng)用的應(yīng)用。細(xì)胞外基質(zhì)蛋白可以混合到水凝膠中并在凝膠化之前非共價(jià)地?fù)饺??;蛘?,具有N-末端半胱氨酸的連接肽也可以與基質(zhì)共價(jià)連接。HyStem水凝膠試劑盒是培養(yǎng)干細(xì)胞的宜選擇,其天然環(huán)境富含透明質(zhì)酸(HA)。HyStem水凝膠可以通過(guò)將細(xì)胞外基質(zhì)(ECM)蛋白或細(xì)胞附著肽添加到水凝膠中來(lái)定制,以提供附著位點(diǎn)和/或分化信號(hào)。它們也可以通過(guò)改變水凝膠的剛性來(lái)改變,以匹配天然細(xì)胞環(huán)境。
特征
· 尺寸(水凝膠頂部的3-D封裝或2-D電鍍)
· 靈活的培養(yǎng)形式(384至6孔板,組織培養(yǎng)插入物和T型瓶)
· 摻入可變量和類(lèi)型的ECM蛋白
· 凝膠特性包括膠凝時(shí)間和水凝膠硬度
無(wú)動(dòng)物成分的系統(tǒng)
,因?yàn)樗膬蓚€(gè)組件是巰基修飾的透明質(zhì)酸(Glycosil HyStem水凝膠是無(wú)異物®)和巰基反應(yīng)性交聯(lián)劑(聚乙二醇二丙烯酸酯,Extralink ® -Lite)。用于生產(chǎn)HyStem的透明質(zhì)酸是通過(guò)使用枯草芽孢桿菌(Novozymes)的專(zhuān)有細(xì)菌發(fā)酵方法制備的。它100%不含動(dòng)物源性原料,并且在其生產(chǎn)中不使用動(dòng)物源性成分。Extralink(聚乙二醇二丙烯酸酯)通過(guò)在聚乙二醇(PEG)聚合物的兩端添加丙烯酸酯基團(tuán)來(lái)制備。PEG衍生自石油和無(wú)機(jī)來(lái)源,不含動(dòng)物源材料。
凝膠化
重構(gòu)的HyStem-C組分在15至37℃下保持液態(tài)數(shù)小時(shí)。當(dāng)交聯(lián)劑,Extralink在水凝膠形成® -Lite(PEGDA)加入到Glycosil的混合物®(巰基修飾的透明質(zhì)酸。所有三種成分混合后,會(huì)發(fā)生在大約二十分鐘膠凝。否步驟取決于低溫或低pH值。用磷酸鹽緩沖鹽水(PBS)或細(xì)胞培養(yǎng)基稀釋組分可以增加凝膠時(shí)間
.3D細(xì)胞回收矩陣
對(duì)于細(xì)胞回收至關(guān)重要的應(yīng)用,替代交聯(lián)劑PEGSSDA可用于所有HyStem水凝膠試劑盒。這種交聯(lián)劑具有Extralink提供的相同優(yōu)點(diǎn),還具有易于還原的內(nèi)部鍵的額外優(yōu)點(diǎn)。這允許從水凝膠中快速,容易地回收單細(xì)胞或簇,用于RNA分析或流式細(xì)胞術(shù)等應(yīng)用,而不是可影響細(xì)胞活力的慢酶法。鼓勵(lì)研究人員與我們聯(lián)系,以確定特定細(xì)胞類(lèi)型或培養(yǎng)系統(tǒng)與PEGSSDA的相容性。
儲(chǔ)存和穩(wěn)定性 | 將Glycosil在-20°C的原始樣品瓶中保存長(zhǎng)達(dá)一年。 |
技術(shù)文件 | GS310技術(shù)數(shù)據(jù)表 產(chǎn)品支持 HyStem Hydrogels常見(jiàn)問(wèn)題解答 小冊(cè)子 HyStem ®水凝膠 |
組件 | Extralink ® |
參考 | 舒,XZ,等。(2004)用于組織工程的原位可交聯(lián)透明質(zhì)酸水凝膠。Biomaterials 25:1339-1348。PMID:14643608. |
ESI BIO產(chǎn)品目錄:
貨號(hào) | 品名 | 規(guī)格 | 品牌 |
GS310 | HyStem® Hydrogel Kit | 2.5 mL | ESIBIO |
GS311 | HyStem® Hydrogel Kit | 7.5 mL | ESIBIO |
GS1004 | HyStem® Hydrogel Kit | 12.5 mL | ESIBIO |
GS310P | HyStem® Hydrogel Kit w/ PEGSSDA | 2.5 mL | ESIBIO |
GS311P | HyStem® Hydrogel Kit w/ PEGSSDA | 7.5 mL | ESIBIO |
GS312 | HyStem®-C Hydrogel Kit | 2.5 mL | ESIBIO |
GS313 | HyStem®-C Hydrogel Kit | 7.5 mL | ESIBIO |
GS1005 | HyStem®-C Hydrogel Kit | 12.5 mL | ESIBIO |
GS312P | HyStem®-C Hydrogel Kit w/ PEGSSDA | 2.5 mL | ESIBIO |
GS313P | HyStem®-C Hydrogel Kit w/ PEGSSDA | 7.5 mL | ESIBIO |
GS314 | HyStem®-HP Hydrogel Kit | 2.5 mL | ESIBIO |
GS315 | HyStem®-HP Hydrogel Kit | 7.5 mL | ESIBIO |
GS1006 | HyStem®-HP Hydrogel Kit | 12.5 mL | ESIBIO |
GS314P | HyStem®-HP Hydrogel Kit w/ PEGSSDA | 2.5 mL | ESIBIO |
GS315P | HyStem®-HP Hydrogel Kit w/ PEGSSDA | 7.5 mL | ESIBIO |
GS1007 | HyStem® Hydrogel UV QuickSet Kit | 2.5 mL | ESIBIO |
GS1008 | HyStem® Hydrogel UV QuickSet Kit | 7.5 mL | ESIBIO |
GS450 | PEGgel Kit | 1 mL | ESIBIO |
GS240 | DG Water | 10 mL | ESIBIO |
GS241 | DG Water | 20 mL | ESIBIO |
GS3007 | Extralink® Vial | 0.5 mL | ESIBIO |
GS3006 | Extralink® Vial | 2.5 mL | ESIBIO |
GS3009 | Extralink® Lite Vial | 0.5 mL | ESIBIO |
GS3008 | Extralink® Lite Vial | 2.5 mL | ESIBIO |
5050 | Fibronectin | 1 mg | ESIBIO |
GS231 | Gelin-S® Thiol-modified Gelatin | 1 mL | ESIBIO |
GS230 | Gelin-S® Thiol-modified Gelatin | 5 mL | ESIBIO |
GS222 | Glycosil® Hyaluronic Acid | 1 mL | ESIBIO |
GS220 | Glycosil® Hyaluronic Acid | 5 mL | ESIBIO |
GS217 | Heprasil® Hyaluronic Acid | 1 mL | ESIBIO |
GS215 | Heprasil® Hyaluronic Acid | 5 mL | ESIBIO |
5010-D | Nutragen® Bovine Collagen | 50 mL | ESIBIO |
GS711 | PEGDA | 1 mL | ESIBIO |
GS700 | PEGDA | 1 g | ESIBIO |
GS705 | PEGDA | 5 g | ESIBIO |
GS755 | PEGSSDA | 0.5 mL | ESIBIO |
5020 | PEPTITE-2000® RGD Peptide | 5 mg | ESIBIO |
5005-B | PureCol® Collagen | 100 mL | ESIBIO |
5007-A | VitroCol® Collagen | 20 mL | ESIBIO |
5051 | Vitronectin | 0.1 mg | ESIBIO |
EM2203 | ExoSense™ CD63 Exosome ELISA Kit | 96 assays | ESIBIO |
EM2202 | VascuNet™ CoCulture Assay Kit | 1 Kit | ESIBIO |
ST11006 | BioLiteTM SSEA-1 (DyLight 488) anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11008 | BioLiteTM TRA-1-81 (DyLight 488) anti-Human Antibody | 100 µL | ESIBIO |
ST11023 | Nestin anti-Human Antibody | 100 µL | ESIBIO |
ST11003 | Oct4 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11001 | Sox2 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11013 | SSEA-1 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11014 | SSEA-3 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11015 | SSEA-4 anti-Human Antibody | 100 µL | ESIBIO |
ST11018 | TRA-1-60 (PE) anti-Human Antibody | 100 µL | ESIBIO |
ST11016 | TRA-1-60 anti-Human Antibody | 100 µL | ESIBIO |
ST11017 | TRA-1-81 anti-Human Antibody | 100 µL | ESIBIO |
ST12007 | LIF, Mouse Recombinant | 10 µg | ESIBIO |
ST12008 | LIF, Mouse Recombinant | 100 µg | ESIBIO |
ES-84 | PureStem® 4D20.8, NCr-fac Progenitor | ea | ESIBIO |
ES-98 | PureStem® E15, Meso-prx/latp Progenitor | ea | ESIBIO |
ES-283 | PureStem® 7PEND24, NCr-fac Progenitor | ea | ESIBIO |
ES-278 | PureStem® 7SMOO32, NCr-fac Progenitor | ea | ESIBIO |
ES-250 | PureStem® SK11, NCr-fac Progenitor | ea | ESIBIO |
ES-268 | PureStem® MEL2, NCr-fac Progenitor | ea | ESIBIO |
ES-256 | PureStem® SM30, NCr-fac Progenitor | ea | ESIBIO |
ES-335 | PureStem® ES-335 Meso-latp Progenitor | ea | ESIBIO |
ES-100 | PureStem® E72 BETATROPHIN+ Progenitor | ea | ESIBIO |
ES-1001 | PureStem® ES-1001 GDF11+ Progenitor | ea | ESIBIO |
ES-184 | PureStem® EN7 Progenitor | ea | ESIBIO |
ES-101 | PureStem® ES-101 Progenitor | ea | ESIBIO |
ES-139 | PureStem® ES-139 Progenitor | ea | ESIBIO |
ES-154 | PureStem® ES-154 Progenitor | ea | ESIBIO |
ES-198 | PureStem® ES-198 Progenitor | ea | ESIBIO |
ES-199 | PureStem® ES-199 Progenitor | ea | ESIBIO |
ES-209 | PureStem® ES-209, Meso-prx/latp Progenitor | ea | ESIBIO |
ES-210 | PureStem® ES-210, Ecto-ntu Progenitor | ea | ESIBIO |
ES-236 | PureStem® ES-236 Progenitor | ea | ESIBIO |
ES-170 | PureStem® E44 Progenitor | ea | ESIBIO |
ES-196 | PureStem® W10 Progenitor | ea | ESIBIO |
ES-194 | PureStem® Z11, Meso Progenitor | ea | ESIBIO |
EM-1001 | PureStem® Progenitor Growth Media, EPM k01 | 500 mL | ESIBIO |
EM-1002 | PureStem® Progenitor Growth Media, EPM k02 | 500 mL | ESIBIO |
EM-1003 | PureStem® Progenitor Growth Media, EPM k03 | 500 mL | ESIBIO |
EM-1004 | PureStem® Progenitor Growth Media, EPM k04 | 500 mL | ESIBIO |
EM-1005 | PureStem® Progenitor Growth Media, EPM k05 | 500 mL | ESIBIO |
EM-1006 | PureStem® Progenitor Growth Media, EPM k06 | 500 mL | ESIBIO |
EM-1007 | PureStem® Progenitor Growth Media, EPM k07 | 500 mL | ESIBIO |
EM-1008 | PureStem® Progenitor Growth Media, EPM k08 | 500 mL | ESIBIO |
EM-2002 | HyStem®-4D Chondrogenesis Differentiation Kit | 1 Kit | ESIBIO |
EM-2007 | HyStem®-4D Differentiation Kit | 1 Kit | ESIBIO |
EM-2001 | PureStem® Chondrogenesis Differentiation Kit | 1 Kit | ESIBIO |
EM-2006 | PureStem® Choroid Plexus Differentiation Kit | 1 Kit | ESIBIO |
EM-2003 | PureStem® Osteogenesis Differentiation Kit 01 | 1 Kit | ESIBIO |
EM-2004 | PureStem® Osteogenesis Differentiation Kit 02 | 1 Kit | ESIBIO |
ST10035 | LY411575 | 5 mg | ESIBIO |
ST10008 | PD0325901 | 2 mg | ESIBIO |
ST10009 | PD0325901 | 10 mg | ESIBIO |
ST10010 | PD0325901 | 415 µL, 10 mM | ESIBIO |
ST10034 | PD173074 | 2 mg | ESIBIO |
ST10021 | RepSox | 5 mg | ESIBIO |
ST10027 | RG108 | 5 mg | ESIBIO |
ST10024 | SB203580 | 2 mg | ESIBIO |
ST10012 | SB431542 | 5 mg | ESIBIO |
ST10013 | SB431542 | 10 mg | ESIBIO |
ST10014 | SB431542 | 1.3 mL, 10 mM | ESIBIO |
ST10025 | SP600125 | 5 mg | ESIBIO |
ST10015 | Thiazovivin | 2 mg | ESIBIO |
ST10017 | XAV939 | 2 mg | ESIBIO |
ST10018 | Y27632 | 2 mg | ESIBIO |
ST10019 | Y27632 | 10 mg | ESIBIO |
ST10020 | Y27632 | 625 µL, 10 mM | ESIBIO |
上海起發(fā)實(shí)驗(yàn)試劑有限公司是實(shí)驗(yàn)試劑一站式采購(gòu)服務(wù)商
1:強(qiáng)大的進(jìn)口輻射能力,血清、抗體、耗材、大部分限制進(jìn)口品等。
2:產(chǎn)品種類(lèi)齊全,經(jīng)營(yíng)超過(guò)700多品牌,基本涵蓋所有生物實(shí)驗(yàn)試劑耗材。
3:提供加急服務(wù),貨品一般1-2周到貨。
4:富有競(jìng)爭(zhēng)力的價(jià)格優(yōu)勢(shì),絕大部分價(jià)格有優(yōu)勢(shì)。
5:多年積累良好的信譽(yù),大部分客戶(hù)提供貨到付款服務(wù)。客戶(hù)包括清華、北大、交大、復(fù)旦、中山等100多所高校,ROCHE,阿斯利康、國(guó)藥、fisher等知藥企。
6:我們還是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等幾十家國(guó)外公司授權(quán)代理。
7:我們還是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知*批發(fā),歡迎合作。